Ameliorating effects of black seed oil on bisphenol A-induced abnormality of blood, hormone profile and gonadal histology of female mice

Bisphenol- A (BPA) is a multi-functional chemical produced in large volumes that is extensively used primarily in the manufacture of polycarbonate plastics and epoxy resins. Black seed oil (BSO) has been employed for many century as one of the main sources of nutrition and healthcare both for humans and animals. The study was carried out to investigate the effects of BPA and BSO on blood, hormone profile, and histopathological changes of ovary in female mice. Total 15 female Swiss Albino mice, aged between 6-8 weeks with an average body weight of 27.4±1g were randomly divided into 3 groups (5 mice/group). Group A act as vehicle control. Group B was administered 50 mg BPA /kg bw daily, while group C received both BPA and BSO and dose rate was 50 mg BPA /kg/day and 1ml BSO/kg/day respectively. Results revealed that Hb concentration, TEC and PCV (p<0.01) were significantly reduced while such difference was absent in other hematological values in BPA treated group compared to control. Inclusion of BSO along with BPA prevented or improved the alterations of those values. Hormonal study showed that, serum thyroxine (T4) and Estradiol level were significantly (p<0.01) reduced by BPA and BSO counteracted the negative effects of BPA. Histopathological study of ovary demonstrated that there were degenerative changes in the lining of granulosa cells of ovarian follicles upon treatment with BPA. From the present study it could be concluded that subjection to BPA is accompanied with anemia, hormonal homeostasis disturbances and granulosa cells degeneration.