PHYTOCHEMICAL SCREENING AND SUBACUTE TOXICITY ASSESSMENT OF DECOCTION OF LIANA BARK OF LANDOLPHIA OWARIENSIS P. BEAUV. (APOCYNACEAE) IN WISTAR RATS

The objective of this study is to search families of chemical compounds and to evaluate subacute toxicity of decoction of Landolphia owariensis liana bark on wistar rat. To identify the family compound chemical staining reactions and / or precipitation was used. Subacute toxicity study was conducted using OECD method 404. 15 rats of both sexes were divided into five groups. They were received for each lot, distilled water (control), 50, 300, 2000 and 5000 mg/kg body weight of decoction every 24 hours orally for 28 days (OECD 404). Body weights, hematological, biochemical and histological parameters of the liver and kidneys were analyzed. The results of phytochemical screening showed that the decoction of L. owariensis contains sterols, polyterpenes, polyphenols and flavonoids. It is rich in chemical substances with antioxidant properties (polyphenols and flavonoids). For toxicity assessment, no significant changes in body weight between control and treated groups and no physiological disturbances were observed after 28 days of treatment. Analyzes of hematological and biochemical parameters revealed no toxicity of the decoction. No mortality was also recorded for the methods used. It is nontoxic to rats, even at high doses (5000 mg/kg body weight). As a result, liana bark of this plant could be used for effective therapeutic purposes.