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Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth

Journal: Journal of Applied Veterinary Sciences (Vol.1, No. 1)

Publication Date:

Authors : ; ; ; ;

Page : 69-72

Keywords : VERO cell; peste des petits ruminants; cell growth;

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This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures; the study proved that the optimum time was 24 hours after subculture of VERO cell line using MOI 2: 1. It was also found that the best time of harvstation of virus fluid of PPR was 9th day post inoculation to reach the best titre 6 log10 TCID50 /ml.

Last modified: 2020-02-25 21:59:26