Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants

ABSTRACT Hyper immune antirabies serum have been prepared in horses using inactivated and live attenuated rabies ERA vaccines with three different adjuvants including 20% Rehydra gel ; 5% Pet gel-A and 20% calcium phosphate gel. Such preparation was conducted through inoculation of each of three horse groups with 8 increased doses (one dose twice weekly) of an inactivated vaccine followed by 4 doses of a live attenuated vaccine (one dose twice weekly) with the same adjuvant. All prepared vaccine formulae were found to be free from foreign contaminants and safe with no post inoculation abnormal signs in inoculated mice. Monitoring of the levels of exhibited rabies antibodies in the sera of immunized horses using serum neutralization test (SNT) and Rabies virus antibody quantitative ELISA kit revealed that the prepared rabies vaccines with 5% Pet gel A adjuvant induced the highest antibody titers (2048 by SNT and 2.20 by ELISA kit) with expected protection percentage 96.14% followed by those induced by 20% Calcium phosphate gel recording the values of 2048 by SNT and 2.11 by ELISA kit with expected protection percentage 92.2%. Lower values (1024 by SNT; 2.05 by ELISA kit and 89.59% protection percentage) were recorded for the Rehydra gel adjuvanted vaccine. These obtained data reflect the safety and potency of the prepared horse anti-rabies hyper immune serum (HARHIS) to be used for post exposure treatment in emergency cases and that is the Pet gel A was the best adjuvant to be used.