Ibuprofen with Sodium Chloride at pH 7.2 and pH 2.4, an In Vitro Interaction Study

Ibuprofen is a important drug which is a nonselective inhibitor of cyclooxygenase, an enzyme involved in prostaglandin synthesis. Its pharmacological effects are believed to be due to inhibition of cyclooxygenase-2 which decreases the synthesis of prostaglandins involved in mediating inflammation, pain, fever, and swelling. Ibuprofen is administered as a racemic mixture and causes vasodilation and depresses myocardial contractility and liver toxicity and kidney dysfunction. Sodium Chloride aqueous is an sodium salt with immune adjuvant activity. Sodium chloride is the salt most responsible for the salinity of sea water and of the extracellular fluid of many multicellular organisms. In the form of edible or table salt it is commonly used as a condiment and food preservative. Large quantities of sodium chloride are used in many industrial processes, and it is a major source of sodium and chlorine compounds used as feed stocks for further chemical syntheses. A second major consumer of sodium chloride is de-icing of roadways in sub-freezing weather. My research study was to investigate the in vitro complexation nature and strength of complex which may be formed due to interaction between Ibuprofen and Sodium Chloride at different pH (7.2 and 2.4) by some physical methods. It was observed that Ibuprofen gives a sharp peak at 272nm when Sodium Chloride mixed with Ibuprofen in 1:1 ratio the intensity of the peak of Ibuprofen change remarkably due to interaction. The Job's plot was obtained by plotting absorbance differences against the mole fraction of the each drug at pH 7.2 and 2.4. Ibuprofen forms strong 1:1 complexes with Sodium Chloride and V shaped curves indicate the formation of 1:1 complexes of Ibuprofen with Sodium Chloride. These may indicate strong kinetics of complexation between Ibuprofen with Sodium Chloride .The value of stability constant for the complexation of Ibuprofen with Sodium Chloride at pH 7.2 and 2.4 were obtained from the spectral data using Ardon's plot. The values of stability constants for the drug-metal system at pH 7.2 and 2.4 are 0.0332 and 0.1018 respectively. It was found that the stability constant for complexation of Ibuprofen & Sodium Chloride system is higher at higher pH then that of Ibuprofen and Sodium Chloride system at lower pH. It can be noticed that a careful consideration is needed during concurrent administration of Ibuprofen with Sodium Chloride.