EFFECT OF SOLVENTS IN CHROMATOGRAPHIC DETERMINATION OF SILDENAFIL CITRATE IN MARKETED FORMULATION
Journal: Indian Drugs (Vol.57, No. 01)Publication Date: 2020-01-28
Authors : Choudhury Priyabati; Roy Smriti; Adhikari Lopamudra; Pattanaik Sovan;
Page : 72-75
Keywords : * For Correspondence E-mail: priyabati04@gmail.com;
Abstract
Chromatography is the procedure for analysing a multicomponent mixture of trace, minor or major constituent by separaationinto its individual fractions.For the analytical determination of sildenafil in pharmaceutical dosages forms an expeditious & sensitive RP-HPLC method is described.The mobile phase composition for chromatographing sildenafil was acetonitrile: methanol: water(60:25:15, V/V/V) with a flow rate of 1mL/min using C18 column. The internal standard selected was racecadotril and its eluents were observed at 217nm. The retention time was 4.919 min for the drug. Between 5-200μg/mL the linearity of this method was observed. For analysis of drug in tablets, the depicted method was found to be appropriate. The results of the analysis was evaluated statistically. The method were found to be robust, accurate and economic with a major advantage of column longevity, as buffer was not used throughout the method. Hence the proposed method can be extended for routine analysis
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