Biosynthesis of calcium oxide nanoparticles using Ocimum sanctum (Tulsi) leaf extracts and screening its antimicrobial activity

In this study, waste egg scale and inexpensive biowaste were employed to remove calcium ions. The potential of egg scales for removal of calcium ions from aqueous solutions was investigated. Preparation of calcinated egg shell powder, tulsi leaf extract, nanoparticle, and growth of bacteria were done to study biosynthesis of calcium oxide nanoparticle onto waste egg scales. Development of green nanotechnology has attracted a great deal of attention from researchers towards eco-friendly biosynthesis of nanoparticle. In this study, biosynthesis of stable calcium nanoparticles was conducted using tulsi (Ocimum sanctum) leaf extract. These biosynthesized nanoparticles were characterized using X-ray diffraction (XRD) analysis. The results revealed that, O.sanctum leaf extract can reduce Ca-ions into calcium oxide nanoparticles within 40 min of reaction time.  It was found that, this method can be used for rapid and eco-friendly biosynthesis of stable calcium oxide nanoparticles with the size ranging from 40 to 70 nm.