DETERMINATION OF ANTIOXIDANT ACTIVITY OF PHASEOLUS VULGARIS PLANT EXTRACTS BY IN-VITRO ANALYSIS

Free radicals produce in body by various environmental and lifestyle changes these days. These free radicals have known to be very unstable species and when formed in high amount can be very dangerous to body and produce condition called Oxidative stress. Oxidative stress has known to be underlying cause for many chronic diseases like cardiovascular disease, Arthritis, Diabetes, cataract, and neurodegenerative diseases. Antioxidants from plant based origin have gained much interest in recent years as they can reduce the harmful effects of oxidative stress without the undesirable side effects. In this approach the plant Phaseolus vulgaris has been selected for determining their antioxidant abilities, crude extract of pet ether, chloroform and methanol were tested. The plants antioxidant potential were determined using DPPH radical Scavenging assay and found that Methanol extract shows the IC50 value of 85.84?0.08 ?g/mL, which shows maximum radical scavenging power, followed by Chloroform and Pet ether. Superoxide radical scavenging assay showed the value of Methanol 46.79?0.09 ?g/mL to be close to standard Ascorbic acid 30.66?0.312 ?g/mL. Pet ether has lowest Superoxide scavenging power than Chloroform. The reducing power assay was determined by making different concentrations of plant extracts and found that as the concentration increases the reducing power of all the extract increases as higher value of absorbance indicates high reducing power. Reducing power follows the order ascorbic acid > methanol > chloroform > Pet ether. The given studies prove the antioxidant capacity of Phaseolus vulgaris plant extracts and further studies can be conducted to explore its application in Therapeutics.