PCR AND ELOCTRON MICROSCOPY BASED DIAGNOSIS OF AN OUTBREAK OF HAEMORRHAGIC SEPTICEMIA IN BUFFALO AND ITS CONTROL IN A FARM OF WEST BENGAL, INDIA

An outbreak of Haemorrhagic Septicemia was investigated in Cattle Re-Settlement Project Farm, Ganganagar, North 24 Parganas, West Bengal, India in the month of December, 2013. Out of 102 nos. of buffalo, 25 animals were affected and 17 were died within 12 - 72 hours. The disease was diagnosed on the basis of history, clinical signs, post mortem findings, histopathological examination, bacteriological study, biochemical tests, biological test, PCR assay and finally by Electron microscopical examination. Antibiotic sensitivity test was done for the isolate. The ailing animals were successfully treated with antibiotic, analgesic and corticosteroid. The epidemic was finally controlled by therapeutic measures, immunization, restriction of movement of animals and proper disposal of carcasses.