Potentially Bioactive New Cu(II), Ni(II) and Co(II) complexes: Synthesis, Spectroscopic, Physico – chemical investigations, DNA Cleavage, Antibacterial and Radical Scavenging Properties

The mononuclear Schiff base Cu(II), Ni(II) and Co(II) complexes have been synthesized using N, N'- (indoline-2,3- diylidene)bis(4-nitroaniline) (L1), and N, N'- (indoline-2,3- diylidene)bis(4-chloroaniline) (L2). The metal complexes have been fully characterized with the help of analytical, conductivity, IR, electronic, and 1H-NMR studies. The molar conductance value indicates that the complex is non-electrolyte in nature. The IR spectra of synthesized compounds confirmed the coordination of ligand to the metal ion. The UV-Vis and magnetic moment data of the complex suggest a distorted octahedral geometry. The Co(II) metal complex and the Schiff base ligand were cleaved supercoiled DNA by oxidative paths, and the nuclease activity of the cobalt complex was assessed by gel electrophoresis assay and the results that metal complex can cleave pUC19DNA in presence of H2O2 compared to the ligands. The antibacterial activities of the synthesized Cu(II) compound and the Schiff base ligand were tested in vitro against the sensitive organisms Staphylococcus aureus, Bacillus subtilis as Gram positive bacteria, Escherichia coli and Klebsilla pneumonia as Gram negative bacteria, and the results are discussed. We found that the antioxidant activity of the Ni(II) complex on DPPH is concentration dependent and the values are higher than that of the Schiff base ligand L1 and L2. The antioxidant activity of the complex was also investigated through the scavenging effect on DPPH