Alfa-Glucosidase inhibitory and antioxidant activity of hexane extract of flowers, leave and stems of Haplophyllum acutifolium DC. and Ferula haussknechtii Wolff ex Rech.
Journal: International Journal of Advanced Biological and Biomedical Research (Vol.8, No. 2)Publication Date: 2020-03-01
Authors : Mohammad Zarei; Hero Ghafaryan;
Page : 153-164
Keywords : Alpha-glucosidase; Ferula haussknechtii; Haplophyllum acutifolium; diabetes; Enzymatic inhibition;
Abstract
Background:
α-Glucosidase Inhibition can significantly prevent glucose uptake after meal, and helps in controlling of some adverse effects in diabetics. So determination of α-Glucosidase inhibitory effect and antioxidant activity of the Haplophyllum acutifolium and Ferula haussknechtii aerial organs was the aim of this study.
Methods:
Inhibitory effect of hexane extracts from different organs was investigated in several concentrations at 405 nm wavelength using a microplate reader. Antioxidant activity of hexane extracts of various organs was also measured using DPPH and iron reduction tests.
Results;
The highest inhibitory activity of F.haussknechtii was observed at the 0.1 g/ml concentration of flower extract (100% inhibition and IC50= 0.1 μg /ml) and the most inhibitory activity of H.acutifolium, was related to the 1 g/ml concentration of flower extract (100% inhibition and IC50 = 10 μg /ml) and leaf extract (100% inhibition and IC50 = 60 μg /ml). Extract of H.acutifolium flower and leaves showed Non-competitive inhibition pattern and F.haussknechtii flower showed mixed (Competitive -Non-Competitive) inhibitory pattern at 0.001 g/ml and exhibit uncompetitive inhibitory pattern at the 0.1 g/ml. The results of antioxidant potential showed EC50 for F.haussknechtii flower and H.acutifolium leaves equalled 2.37 and 0.96 mg/ml, respectively.
Conclusions:
The hexane extract of the F.haussknechtii flower, and H.acutifolium flower and leaf organ have a significant inhibitory effect on the activity of α-Glucosidase, DPPH free radical scavenging activities and reducing power. So they are good resources for extraction of medicinal compounds to control blood level of glucose after meal, in diabetic patients.
Other Latest Articles
- Effect of crop residue and nitrogen levels in yield and yield attributing traits of rice under Rice-Wheat Cropping System
- The recent development in synthesis and pharmacological evaluation of small molecule to treat Alzheimer's diseases: A review
- Introduction of Microbial as a Major Factor in the Human Body
- The effect of halogen atoms at propanoate anion on thermo physical, vibrational spectroscopy, chemical reactivity, biological properties of morpholinium propionate Ionic Liquid
- Histamine and Food Allergy
Last modified: 2020-05-08 17:11:13