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APPLICATION OF UV-SPECTROPHOTOMETRY FOR DETERMINATION OF ZILEUTON IN BULK AND IN TABLETS

Journal: Indian Drugs (Vol.50, No. 6)

Publication Date:

Authors : ; ;

Page : 40-44

Keywords : *Email: atulshirkhedkar@rediffmail.com;

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Abstract

Zileuton is an orally active inhibitor of 5-lipoxygenase and found to inhibit leukotrienes (LTB4 , LTC4, LTD4 , and LTE4) formation and well-known for the effective treatment of asthma. Rapid, economical and simple ‘Zero order UV-spectrophotometry' and ‘First order derivative UV-spectrophotometry' have been developed for estimation zileuton in bulk and in-house tablets. Zileuton showed maximum absorbance at 258.4 nm in 0.01M NaOH. For Zero order UV-spectrophotometric method (Method I), two wavelengths 243.4 nm and 271.0 nm were selected for the determination of area under curve (AUC). In First order derivative UV-spectrophotometry (Method II), two wavelengths 260.0 nm and 279.60 nm were selected for determination of AUC. For both these methods, zileuton followed linearity in the concentration range of 4 - 24 µg/ mL with correlation coefficient value of 0.999. Both methods were validated as per ICH guidelines.

Last modified: 2020-05-28 20:38:04