Forced Degradation Study of Eletriptan by Using Ultra High Performance Liquid Chromatography (UHPLC)
Journal: Indian Drugs (Vol.50, No. 7)Publication Date: 2013-07-28
Authors : Sahu S. Singh R.M Mathur S.C. Sharma D. K. Saini P.K. Duggal D. Tyagi V.; Singh G. N.;
Page : 14-21
Keywords : *E-mail: raman19662002@gmail.com;
Abstract
A simple, fast, precise and accurate ultra high performance liquid chromatography method was developed for degradation study of eletriptan hydrobromide (EH) under exaggerated conditions. An Inertsil ODS C18 (250 x 4.6 mm, 5µm) column in isocratic mode was used with mobile phase comprising of water, methanol and trifluoroacetic acid mixed in the ratio 55:45:0.1 % V/V/V, maintained at pH 3.5. The flow rate was set at 0.4 mL per minute with UV detection at 225 nm. The retention time of EH was found to be 3.7 minutes. Linearity for EH was found in the range of 3.5- 200 µg per mL and percentage recoveries were obtained in the range of 100.2 % to 100.6 %. The method was capable of resolving all degradants and principle component in sample. The proposed method is accurate, precise, selective, reproducible, and rapid for detection of degradation of eletriptan hydrobromide.
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