A VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PANTOPRAZOLE AND CINITAPRIDE IN A PHARMACEUTICAL FORMULATION
Journal: Indian Drugs (Vol.51, No. 1)Publication Date: 2014-01-28
Authors : Dighade N. R. Padmane S. P.; Kasture A. V.;
Page : 27-33
Keywords : *E-mail: narendra_dighade@rediffmail.com;
- A VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PANTOPRAZOLE AND CINITAPRIDE IN A PHARMACEUTICAL FORMULATION
- VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PRAVASTATIN AND FENOFIBRATE IN PHARMACEUTICAL DOSAGE FORMS
- VALIDATED RP - HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ATORVASTATIN AND EZETIMIBE IN PURE AND COMBINED PHARMACEUTICAL DOSAGE FORMS
- A VALIDATED STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CEFPODOXIME AND LEVOFLOXACIN IN BULK AND FORMULATION
- Stability Indicating Validated RP-HPLC Method Development for Simultaneous Estimation of Benidipine Hydrochloride and Telmisartan from Pharmaceutical Dosage Form
Abstract
The study describes a validated stability indicating reverse- phase HPLC method for the simultaneous estimation of pantoprazole and cinitapride in capsule formulation. The proposed RP-HPLC method utilizes an Eclipse XDB C18 Column (150 × 4.6 mm i.d., 5μm), optimum mobile phase consisting of 10 mM phosphate buffer: acetonitrile: THF in the ratio of 64:36:0.5 V/V (pH 3.5) V/V, effluent flow rate 1 mL/min and UV detection wavelength of 266 nm. The selected chromatographic conditions were found to effectively separate pantoprazole and cinitapride with retention time of 7.17 min and 3.56 min, respectively. Linearity for pantoprazole and cinitapride was found in the range of 2-30 μg/mL and 0.15- 2.28 μg/mL, respectively. The developed method was statistically validated for the linearity, accuracy, precision, robustness, ruggedness and specificity. The proposed method was found to be simple, accurate, precise, economical and specific. Therefore, it can be used for simultaneous analysis of these drugs in capsule formulation.
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