DEVELOPMENT OF HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF OFLOXACIN AND ORNIDAZOLE IN COMBINED DOSAGE FORM
Journal: Indian Drugs (Vol.51, No. 4)Publication Date: 2014-04-28
Authors : Dighade N. R. Shende M. D.; Kasture A. V.;
Page : 20-25
Keywords : *E-mail: narendra_dighade@rediffmail.com;
Abstract
A simple and accurate high performance thin layer chromatographic (HPLTC) method have been developed and validated as per ICH guidelines for estimation of Ofloxacin (OFL) and Ornidazole (ORN) in combined dosage form. The mobile phase was acetone : water : toluene : triethylamine (5.8:1.5:2:1.6 V/V) was found to be best which gave high resolution with Rf 0.25 and 0.82 for ofloxacin and ornidazole respectively. The linearity of ofloxacin and ornidazole was found to be in the range of 0.2 to 0.3 µg/mL and 0.4 to 0.8 µg/mL respectively. The coefficient of correlation (r2 ) was found to be greater than 0.9948 for both the components by this method. The tablet analysis result (n = 5) were close to 100 % by HPTLC for both the components. The proposed method was found to be simple, accurate and suitable for routine quality control of marketed formulation containing these drugs.
Other Latest Articles
- Publication activity of Russian researchers: academic science
- PEGYLATION: CONCEPT AND APPLICATIONS IN CANCER THERAPEUTICS
- The results of monitoring of Russian core shared research facilities and unique scientific installations for 2015
- QUALITATIVE ASSESSMENT OF PARADA BHASMA (CALX) USING NAMBURI PHASED SPOT TEST
- FORMULATION AND EVALUATION OF DIRECTLY COMPRESSIBLE PIOGLITAZONE HYDROCHLORIDE TABLETS BY INCORPORATING DRUG SPHERICAL AGGLOMERATES
Last modified: 2020-06-09 19:46:42