Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Adipose-derived stem cells (ADSCs) are wonderful cells for regenerative medicine. Like mesenchymal stem cells, ADSCs hold multi-potent differentiation capacity that can differentiate into osteoblasts, chondrocytes and adipocytes, as well as trans-differentiation into some other cells. ADSC transplantation rapidly increased for recent years, especially in vitro expanded ADSC transplantation. This study aimed to provide a new method to in vitro primarily culture and secondary culture of ADSCs that were compliant with good manufacturing practice for clinical applications. Stromal vascular fraction (SVF) was extracted from adipose tissue by commercial kits. SVF was expanded in vitro in medium with non-allogeneic supplements. Cultured ADSCs maintained immune-phenotype, karyotype, and differentiation potential after ten passages. Moreover, ADSCs at 10th passage could not form tumors in NOD/SCID mice. This research contributed a suitable protocol for clinical applications of expanded ADSCs.