COMPARATIVE IN VITRO ANTIDIABETIC ACTIVITY OF PRUNUS AMYGDALUS (BAIL.) AND HIBISCUS ROSA-SINENSIS (LINN.)

A comparative study was designed to evaluate the in vitro antidiabetic activity from the aqueous extract of dried pericarp of Prunus amygdalus Bail and ethanolic extract of dried flower of Hibiscus rosa-sinensis L. against the standard Acarbose.The aqueous extract of P. amygdalus and ethanolic extract of H. rosa-sinensis were extracted by the continuous hot extraction process.The qualitative phytochemical screening of aqueous extract and ethanolic extract reveals the presence of phytosterols, phenols, carbohydrates, flavanoids, saponins, amino acids and alkaloids, phenol, tannins, cardiac glycoside, saponin and flavonoids, respectively. In vitro pharmacological activity of P. amygdalus and H.rosa-sinensis were performed and compared by α-amylase inhibition assay method. The percentage inhibition of α-amylase by P. amygdalus, H. rosa-sinensis and Acarbose (50 &100 μg/mL) at dose of 50mg/mL and 100mg/mL were found to be 60.11±2.74%,74.86±0.31*%, 71.36± 1.86%, 81.49±1.72%, 82.33±1.10% and 95.37±0.56*%, respectively. The IC50 values were found to be 40.26μg/mL for acarbose, 49.63mg/mL for ethanolic extract of H. rosa-sinensis and 56.42mg/mL for aqueous extract of P. amygdalus.