Ceftriaxone with Antacid and Metal Complexation and Investigation of Antimicrobial Activity, In-Vitro Demonstration

The research work comprises of interaction studies of ceftriaxone with essential metal, antacid and investigation of antimicrobial activity of ceftriaxone. Ceftriaxone is included among the cephalosporins drug class which is active against a wide range of gram positive and negative bacteria. Since the presence of compelling ligand may affect the bioavailability of a metal in the blood or tissues, therefore in order to study the portable interaction of ceftriaxone with essential and trace elements present in the body. Ceftriaxone has been interacted with Zn (metal), Ca (antacid) as an in-vitro analysis. All the reaction conditions were simulated to natural environment. Also the anti-microbial activity of the drug and the complexes were determined. There is an effect of pH on drug metal complexation. It has observed that ceftriaxone interacts with metal on a pH 7.4, the stability constant of these complexes were determined in order to evaluate their possible in vivo implications. This research work confirms that there was a possible interaction between the ceftriaxone and metal Zn and antacid Ca which was confirms by jobs plot method and by antimicrobial investigation it was confirmed that the zone of inhibition of ceftriaxone with Metal Zn and antacid Ca reduced from 16 mm to 14mm & 11mm respectfully. The standard ceftriaxone disk also tested against Staphylococcus aureus. In order to investigate the number of metal ions involved in the complexation with ceftriaxone complexes were elucidated by plotting various UV spectrophotometric methods. The ultraviolet studies of these complexes were carried out and compared.