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Use of Ghee Residue As A Substrate For Microbial Lipase Production

Journal: International Journal of Scientific & Technology Research (Vol.1, No. 10)

Publication Date:

Authors : ; ; ; ;

Page : 61-64

Keywords : Keywords- Lipase; ghee residue; Tributyrin oil; SSF; SLF;

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Abstract

ABSTRACT - Microbial lipases are commercially important and can be produced by Solid State Fermentation SSF as well as by Submerged Liquid Fermentation SLF. In this study ghee residue which is one of the largest by-product of dairy industry was used as a substrate. The main objective of the study was to determine whether ghee residue could be used as a substrate over the conventional synthetic substrate for efficient lipase production. Two organisms were used for lipase production one of them was a known lipase producer - Bacillus subtilis NCIM 2063 and the other organism belonging to the Proteus spp was isolated from the soil contaminated with oil. The production of lipase was significantly higher in SSF ghee residue as substrate than in SLF tributyrin oil as substrate. The lipase yield was observed to be 41.27 Umg by Proteus spp and 35.93 Umg by B. subtilis in SSF using ghee residue as substrate. In SLF using Tributyrin as substrate the lipase production by Proteus spp was 20.78 Umg and 28.63 Umg by B. subtilis.

Last modified: 2013-04-13 21:50:05