Phytoconstituent Analysis and Antibacterial Potential of Epicarp Extracts from Mature Fruits of Persea americana Mill
Journal: Molecular and Cellular Biomedical Sciences (Vol.4, No. 2)Publication Date: 2020-07-01
Authors : Cyuzuzo Callixte Dusabimana Jean Damascene Anwar Ma'aruf Yoes Prijatna Dachlan Anggraini Dwi Sensusiati Ndayisaba Daniel Eka Nora Vitaloka Aprilia Putri Winthoko;
Page : 94-9
Keywords : antibacterial activity; ethanol extract; chloroform extract; Persea americana; Rwanda;
Abstract
Background: World Health Organization (WHO) has reported the antimicrobial resistance as one among the ten threats to global health in 2019. The development of plant-derived antibiotics is currently considered as a modern medicine's greatest success. Persea americana is a plant with high medicinal profile which allow its different parts to be used for therapeutic purposes. This study is aimed to determine the antibacterial potential of ethanol and chloroform extracts from epicarp of mature fruits of P. americana Mill against human pathogens. Materials and Methods: The epicarps of avocado were dried in oven and ground into powder using porcelain mortar and pestle. The powdered plant materials were extracted with both 96% ethanol and chloroform. Extracts were qualitatively screened to examine their bioactive contents and agar well diffusion method was used to analyze the antibacterial activity of extracts against both Gram-positive and Gram-negative bacteria. Results: Both solvents showed the ability to dissolve the secondary metabolites from avocado epicarps. Phytochemical screening disclosed the presence of alkaloids, proteins, terpenoids, tannins, flavonoids, steroids and phenolic compounds in ethanolic extracts and absence of flavonoids and tannins in chloroform extracts. The extracts showed the inhibition zones ranging from 14±4.5 mm to 26±2.1 mm while streptomycin demonstrated high inhibition zones ranging from 20±3.1 mm to 30 mm. The minimum inhibitory concentration (MIC) values of extracts fall in the range of 0.3125 mg/mL and 20 mg/mL while the MIC values for streptomycin vary from 0.25 mg/mL to 1.25 mg/mL. Conclusion: The ethanol and chloroform extracts proved to be potentially effective and to be used as natural alternative preventives to fight against various disease-causing bacteria.
Other Latest Articles
- The Mechanism of Coronary Artery Calcification in Centrally Obese Non-Diabetic Men: Study on The Interaction of Leptin, Free Leptin Index, Adiponectin, hs-C Reactive Protein, Bone Morphogenetic Protein-2 and Matrix Gla Protein
- High Blood Ammonia Levels Associated with Long-term Valproic Acids Therapy in Epileptic Children
- Isolation, Characterization, Proliferation and Differentiation of Synovial Membrane-derived Mesenchymal Stem Cells (SM-MSCs) from Osteoarthritis Patients
- Comparison of Antiaging and Antioxidant Activities of Protocatechuic and Ferulic Acids
- Density of Dermatophagoides spp. and Its Relationship with House-dust Mite Specific Serum IgE in Persistent Asthma
Last modified: 2020-07-02 12:54:08