A NEW RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF FLOUXAMINE, CHLOMIPRAMINE HCL IN ITS BULK AND PHARMACEUTICAL DOSAGE FORMS

The objective of the current study was the development of a simple, precise and accurate isocratic reversed-phase High Performance Liquid Chromatography [RP-HPLC] assay method and validated for determination of Flouxamine and Chlomipramine Hcl in capsule dosage forms. Isocratic separation was achieved on a C18 Inertsil ODS (5μ, 250mm× 4.6mm) with flow rate of 1.0ml/min using UV detection at 230nm. Different mobile phase compositions of pH 3.5 phosphate buffer: ACN (30:70) in order to determine the best conditions for the effective separation and elution of the analytes. The mobile phase consisting of pH 3.5 buffer: ACN (30:70) was selected. The injection volume was 10.0 μl and the detection was carried out at 254 nm by using photo-diode array detector. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The proposed method was successfully used to determine the drug content of marketed formulation.