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A NEW RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF FLOUXAMINE, CHLOMIPRAMINE HCL IN ITS BULK AND PHARMACEUTICAL DOSAGE FORMS

Journal: Indo American Journal of Pharmaceutical Sciences (IAJPS) (Vol.1, No. 3)

Publication Date:

Authors : ; ; ;

Page : 163-170

Keywords : Flouxamine; Chlomipramine Hcl; RP-HPLC; Methanol and Acetonitrile;

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Abstract

The objective of the current study was the development of a simple, precise and accurate isocratic reversed-phase High Performance Liquid Chromatography [RP-HPLC] assay method and validated for determination of Flouxamine and Chlomipramine Hcl in capsule dosage forms. Isocratic separation was achieved on a C18 Inertsil ODS (5μ, 250mm× 4.6mm) with flow rate of 1.0ml/min using UV detection at 230nm. Different mobile phase compositions of pH 3.5 phosphate buffer: ACN (30:70) in order to determine the best conditions for the effective separation and elution of the analytes. The mobile phase consisting of pH 3.5 buffer: ACN (30:70) was selected. The injection volume was 10.0 μl and the detection was carried out at 254 nm by using photo-diode array detector. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The proposed method was successfully used to determine the drug content of marketed formulation.

Last modified: 2014-10-08 17:57:17