BACTERIAL ISOLATED AND DIAGNOSIS FROM THE RESPIRATORY SYSTEM OF SMOKERS AND NON SMOKERS INDIVIDUAL AND COMPARE THE BACTERIA WITH THE ISOLATED BACTERIA FROM SOME TYPES OF CIGARETTES WHICH COLLECTED FROM THE MARKETS AND MEASURES

Fifty sample was collected and divided into three groups, the first twenty (20) of them collected from the respiratory system of smokers individual, and the second twenty (20) samples was collected from the respiratory system of non smokers individual, and the latest group collected from ten types (10 type) of cigarettes which were used in markets by smokers in Al Muthana City in Iraq. The first and second of the collected samples groups had been collect by coughing on sterile Petri dishes with nutrient agar and then incubated in 37C° for 24 hour to see the colonies growth of bacteria to isolate and diagnosis. In addition for that we collected a blood samples from these groups to measures the differentiations in hemoglobin range and blood viscosity in both groups individuals. On the third groups of samples which are ten samples of cigarettes (10 types of cigarettes) which collect from the markets which were (Aspin, Royal, Oscar, Pine, Blue Bon, White Bon, Master, Marlboro, Gauloises , kent ) which the tobacco of each type has been collected in separate test tube and soaked with distal water for 24 hour and then taken 1 ml of the clear liquid to culture it on sterile Petri dishes with nutrient agar and then incubated in 37C° for 24 hour to see the colonies growth of bacteria to isolate and diagnosis. We detect that the first group of samples (smokers individual) were infect with a dense growth of bacterial colonies of Staphylococcus aureus and Staphylococcus albus which may reach to 80% of the summation collected samples which may cause abscess and necrotic pneumonia and may be lead to death. Also we detected there are Clostridium perfringens and Clostridium botulinum bacteria with 55% of the collected samples these bacteria may be lead to gas gangrene in pulmonary tissue. In addition we detected there are Streptococcus pyogen which reach to 35% of the collected samples these bacteria may cause septic inflammations. So the second group of sample appear that there are colonies growth of Staphylococcus supp. With 65%, Streptococcus supp. With 10% , Clostridium supp