SCREENING AND CHARACTERIZATION OF HEXAVALENT CHROMIUM DEGRADING BACTERIA FROM INDUSTRIAL SAMPLES
Journal: International Journal of Advanced Research (Vol.8, No. 7)Publication Date: 2020-07-14
Authors : Gunti Vijay Kumar; Pandu Brahmaji Rao;
Page : 222-228
Keywords : Bacillus Subtilis Chromium (VI) Degradation Industrial Effluent Polymerase Chain Reaction (PCR);
Abstract
Industrial wastes are capable of polluting the soil and groundwater, are taken into account as a possible environmental polluting agent throughout the earth. Chromium (Cr), a virulent serious metal, could be a major constituent of industrial waste, and its accumulation in soil and water causes serious environmental problems with increasing public concern in India mainly in Telangana. This study deals with isolation and identification of Cr resistance microorganisms isolated from industrial effluents collected from Uppal region, Hyderabad. Cr resistance microorganism was isolated and the identification of the isolates was done by 16s rRNA sequencing followed by BLAST results confirming the novel Bacillus subtilis strain. Moreover, the presence of Chromium reductase enzyme within the isolated microorganism was confirmed by PCR techniques. Bioremediation by microorganisms might offer an alternate or aid standard strategies of Cr metal removal capacity. The novel is known as Cr resistance microorganism would be helpful for the detoxification of heavy metal contaminated industry effluent and metal recovery in the mining method.
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