Detection and Identification of Helicobacter pylori from Patients and Comparison of Different Methods

The aim of this study was to determine the most appropriate method for identification of Helicobacter pylori following isolation from gastric mucosal biopsy of patients with clinical signs of gastritis or duodenitis. Microbiological analysis and polymerase chain reaction (PCR) were found to be the most appropriate and highly reliable in diagnosing the presence of H. pylori in the gastrointestinal mucosa in patients with clinical symptoms of gastritis or ulcer. The microbiological analysis of 41 gastric mucosal biopsy specimens from patients with clinical symptoms of gastritis or ulcer revealed H. pylori in 36 patients (87.80%), mixed H. pylori and С. jejuni culture in four patients (9.75%) and presence of lactic acid bacteria in just one patient (2.45% of cases).