Influence of Enzymаtically Hydrolysed Polysaccharide from Plantago Major L. on BetaXylosidase Activity in Lactobacillus Strains

The dynamics of hydrolysis of total acid-extractable polysaccharides from leaves of the medicinal plant Plantago major L. using hemicellulase and xylanase were studied. The degree of polysaccharide hydrolysis and the monomeric units were experimentally determined. Hemicellulase and xylanase hydrolysis both indicated larger amounts of detected monosaccharides with increasing the reaction time. The enzymatic hydrolysis conducted with hemicellulase showed a higher degree of hydrolysis (93.7% after 28 h) compared to the hydrolysis carried out with the enzyme xylanase (27.8% after 28 h). Galactose was the prevalent monosaccharide identified by HPLC analysis. It could be proposed that in the total acid-extractable polysaccharides from P. major leaves a smaller number of the monomer residues are linked by β-1,4-glycosidic linkages. Probably β-1,3 or β-1,6-linkages prevail, which are the basis of arabinogalactan II, or α-1,4-linkages, building pectic type polysaccharides. Our results from the enzymatic reactions demonstrated the in vitro capacity of β-xylosidase from L. plantarum S26 and L. brevis S27 to hydrolyse xylobiose and polysaccharide hydrolysates from P. major leaves.