Oligosaccharide Production from Lactose and Lactulose by β-Galactosidase from Lactobacillus plantarum S30

A β-Galactosidase preparation from ten strains Lactobacillus plantarum was evaluated as a biocatalyst for the synthesis of oligosaccharides. Among them, the enzymes from L. plantarum S26 and L. plantarum S30 were selected for further studies. The correlation between organic acid production, α-galactosidase, β-galactosidase and lactate dehydrogenase activity and the initial concentration of lactose and lactulose was studied. It could be suggested that the studied β-galactosidase catalyzes the trans-glycosylation reaction at 20% lactose and 20% lactulose concentrations.