Effect of Different Concentrations of Calcium Silicate on the in vitro Rooting of Date Palm Shoots cv. Sewi

The current study focused on the effect of different concentrations of Calcium Silicate (CaSiO3), Fe-EDTA and thiamine HCL added to culture medium at the appropriate subculture during rooting stage in order to get rapid and satisfied adventitious rooting and subsequently increase the survival percentage of the acclimatized plants in the greenhouse. This work describes the major advances of morphological and physiological differences in date palm plantlets Sewi cultivar with different concentrations of calcium silicate during the in vitro rooting stage. Four treatments of CaSiO3 were tested through being added to MS medium at the concentrations 0, 2, 4, 8, and 16 mg/l with 0.1 mg/l NAA and 0.5 mg/l GA3. The results showed that medium containing 2.0 mg/l and 4.0 mg/l CaSiO3 promoted the plantlets development as well as the highest rooting. Supplementation of culture medium with 4.0 mg/l CaSiO3, 0.1 mg/l NAA, 0.5 mg/l GA3, 35 g/l sucrose, 0.15 g/l Fe-EDTA and 2.5 mg/l Thiamine HCL with ½ MS basal medium for 8 weeks of subculture significantly increased length of the plantlets and improvement of roots. There was an increase in levels of chlorophyll a, b and in addition to total protein content in the presence of calcium silicate.