Effects of Different Proportions of Salvianolic Acid and Hydroxysafflor Yellow A on the Myocardial Ischemia Model Induced by Pituitrin in Rats

Aim: In this experiment, SD rats were injected intravenously with different proportions of test samples to observe the protective effect of intravenous injection of pituitrin (PIT)-induced myocardial ischemia in rats, and to determine the lipid peroxidation product of Malondialdehyde (MDA) in rat brain and the content and activity of Superoxide Dismutase (SOD), Creatine Kinase (CK) and Lactate Dehydrogenase (LDH). The role of the anti-myocardial ischemia model in the test and its optimal ratio were studied. Materials and methods: Experimental SD rats sensitive to pituitrin were randomly divided into normal saline group, model group, positive control group, and administration group (7 groups). Each group of test samples was injected through the tail vein and the lead ECG was traced. After administration for 30 min, the rats were sublingually injected with 2U/kg pituitrin, and Ⅱ lead ECG was recorded for 5 min. After 3 hours of observation, the rats were sacrificed from the cervical spine, and the hearts were removed and placed in a refrigerator at -20°C to measure various biochemical indicators. Results: The contents of MDA, LDH, and CK in myocardial ischemia model group were significantly higher than those in saline control group, and SOD activity was significantly decreased. Intravenous injection of each group of tested products has the effect of reducing the content of MDA, LDH, and CK in myocardial tissue, which can enhance the activity of SOD in myocardial tissue, and has statistical significance compared with the model group (P≤0.05 or P≤0.01). In a comprehensive comparison, the A:B=1:10 dose group had the best results. Conclusion: Under the experimental conditions, each dose group of the test article can significantly reduce the levels of MDA, LDH, and CK in the myocardial tissue of ischemic rats, and increase the activity of SOD. The effect of this dose has an increasing trend with the increase of B components, and has a certain degree of Dose-effect relationship.