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Dynamics of Activity of Aspartate Aminotransferase in Blood Plasma of Goats During Pregnancy

Journal: Sumerianz Journal of Scientific Research (Vol.1, No. 2)

Publication Date:

Authors : ;

Page : 48-51

Keywords : Aspartate Aminotransferase; Blood Plasma; Goats.;

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Abstract

In the work presented, data on the activity of the enzyme aspartate aminotransferase in the blood plasma of domestic goats during pregnancy are given. Determination of the activity of the enzyme aspartate aminotransferase was performed spectrophotometrically on a Specol 1500 Analitik Jena (USA) spectrophotometer using the method of Bergmeyer H.U., Hoder M., Rej R. The optical density of the samples was measured at a wavelength of 540 nm. The activity of aminotransferases was also determined by the improved Raitman method proposed by Kolb VG, Kamyshnikov VS and Osadchaya L.M. AST enzyme activity in the blood plasma during the first third of pregnancy was 85,50 ± 5,25 U/L. In the second third of pregnancy AST activity averaged 95,20 ± 3,36 U/L. U/L. During the final third of pregnancy, decrease of AST activity in goat blood plasma was recorded, averaging 72,00 ± 1,98 U/L. Thus, a significant decrease in ACT activity was determined in the last third of the gestation period compared to the first and second third. The results obtained can serve for better understanding of the biochemical processes in pregnant goats, to assess their physiological status and diagnostic purposes.

Last modified: 2020-08-15 19:58:52