GC-MS PROFILE, ANTIBACTERIAL, ANTIFUNGAL, AND ANTICANCER ACTIVITY OF ROOT OF VANDA TESSELLATA AN EPIPHYTIC ORCHID

Background:The presence of phytochemical constituents has been reported from species of the Orchidaceae. Hitherto no reports exist on the GC-MS Profile of Vanda tessellata Hook. Ex G. Don which was an epiphyte from the sacred groove of Penchalikona of Andhra Pradesh. Objective:The current study was to determine the antibacterial, antifungal,anticancer activities, and GC-MS profiles of the root of Vanda tessellata Hook. Ex G. Don. Materials and Methods:The root of the Vanda tessellata Hook. Ex G. Don which was an epiphytic orchid from the sacred groove of Penchalikona of Andhra Pradesh. An in-vitro evaluation of antibacterial and antifungal activity was performed by disc diffusion method and dilution technique, and anticancer activity was performed by SAB assay. Gas chromatography-mass spectrometry (GC-MS) analysis of the root extracts of Vanda tessellata Hook. Ex G. Don was performed on GC-MS equipment (GCMSQP2010, SHIMADZU). Results:The GC-MS profile has shown the presence of different phytochemical compounds in the epiphytic root of Vanda tessellata Hook. Ex G. Don. A total of 33 compounds were identified. Furthermore, the results of antibacterial and antifungal tests (both primary and secondary) shown that substantial antibiotic activity, and the anticancer activity results are shown inactivity. Conclusion:From the results, it is evident that epiphytic root extracts of Vanda tessellata Hook. Ex G. Don has shown substantial antibacterial, and antifungal activity and GC-MS profile revealed the 33 analytes confirming the root was a source of therapeutic compounds useful for the pharmaceutical applications.