Optimization of Slight Halotolerant α-Amylase Producing Brevibacillus Borstelensis R1 in Various Media by Submerged FermentationJournal: International Journal of Engineering Sciences & Research Technology (IJESRT) (Vol.3, No. 9)
Publication Date: 2014-09-30
Authors : K.Suribabu; K.P.J Hemalatha;
Page : 289-295
Keywords : Brevibacillus borstelensis R1; alpha-amylase; media; slight halotolerant.;
Bacteria in marine habitat have modified structure of enzymes, ribosomes, and transport proteins which require high levels of potassium for stability and activity. In the present study, α-amylase producing Brevibacillus borstelensis R1 was isolated from marine waters of Bay of Bengal, Rusikonda, Visakhapatnam by applying different primary screening techniques. The secondary screening of α-amylase production of Brevibacillus borstelensis R1 was carried out at varying % of NaCl using ten different media. The sodium chloride response towards 1.0%, 2.0%, 3.0% and 4.0% was positive and the response was negative at 5.0% NaCl. B.borstelensis R1 was categorized under slight halophile which shows optimum growth at 2?5% NaCl. Alpha-amylase produced by B.borstelensis R1 have many applications in starch processing, desizing of textiles, paper sizing, detergent additive, bread improvement, ethanol production, sewage treatment, effluent treatment and other fermentation processes.
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Last modified: 2014-10-16 21:08:08