Genetic Study of Methicillin Resistant S. aureus (MRSA) Isolated from Neonatal Infections

This study aimed to detect of methicillin resistant S. aureus (MRSA) which caused neonatal infections and to detect the genes being responsible of antibiotic resistance in these bacteria by molecular technique (PCR). Antibiotic sensitivity test results demonstrated that, S. aureus isolates were highly resistant against most antibiotics, 100% for each AMP, AMC, CTX, CTR, CAZ, KF and K. whereas it's resistance ?70% for most other antibiotics. While 0% resistance (100% sensitivity) was shown in each IPM, MRP. The results of minimum inhibitory concentration MIC by using HiComb test showed that S. aureus has 100% resistance for both MET and OX with MIC values ranged between (5-240μg/ml and 32-256μg/ml respectively). While 0% resistance reported for VA (0.024-0.512μg/ml). PCR results demonstrated that, among 35 isolates of S. aurus were determined previously as MRSA by phenotypic methods, 91.4% have mecA gene, 37% have nuc gene and 2.9% have vanA.