Standardization of Efficient Indirect Plant Regeneration Protocol in Brinjal (Solanum melongena L.)

The auxins viz. 2, 4-D, NAA alone or in combination to BAP (1.0mgl?1) did not induce good quality callus. More than 1ppm IBA formed less and quite compact callus at cut ends with rooting only after two weeks. IBA (0.5-2.0 mgl?1) + 1.0 mg/l BAP increased the mass of pale white, compact, nodular callus with embryogenesis in ten days that differentiated on the callusing media. With IBA and BAP, hypocotyls induced 100.00% callus on all the MS media combinations (1.5mgl?1 IBA + 1.0mgl?1 BAP, 1.0mgl?1IBA +1.0 mgl?1BAP and 0.5mgl?1IBA + 1.0mgl?1BAP), while cotyledons produced maximum callus on MS with 1.5mgl?1IBA + 1.0mgl?1BAP (97.03%). However, cotyledon induced 95.64% somatic embryogenesis on MS +1.5mgl?1 IBA + 1.0mgl?1 BAP followed by leaf (94.10%). Hypocotyl had no somatic embryogenesis on MS media fortified with conc. of 1.5 mgl?1IBA + 1.0mgl?1BAP, which could be increased with further decrease in IBA levels. Callus induced by NAA, 2,4-D and IBA auxins suppressed shoot bud initiation, while IBA +BAP induced callus diverted towards differentiation into shoots on higher levels of BAP+ kin. Furthermore, organogenesis was not observed in the callus induced from the hypocotyl, whereas it was maximum in cotyledon (55.02%) followed by leaf (44.57%) on MS medium supplemented with 2.5mgl?1BAP + 1.0mgl?1kin + 0.2% activated charcoal.