Immunohistochemical study of P53 protein expression in different prostate cancer Gleason grading groups
Journal: RUDN Journal of Medicine (Vol.24, No. 2)Publication Date: 2020-05-27
Authors : G. Kudryavtsev; L. Kudryavtseva; L. Mikhaleva; Y. Kudryavtseva; N. Solovyeva; V. Osipov; I. Babichenko;
Page : 145-155
Keywords : Ki-67; P53; prostate cancer; adenocarcinoma; Ki-67; P53; Gleason index;
Abstract
Prostate cancer (PC) remains an urgent public health problem, especially in developed countries. The use of immunohistochemical research methods in addition to the morphological classification of prostate adenocarcinomas allows a more accurate diagnosis and prognosis of the disease. The aim of the study is to identify isoforms of P53 using clones of mouse antibodies (D-07 and Y5; Epitomics, USA) in prostate cancer with different proliferative activity and the degree of malignancy. Materials and Methods: The work included surgical material for prostate resection and prostatectomy, as well as biopsy specimens (56 cases in total). An immunohistochemical study was carried out with the Ki-67 marker, as well as with mouse monoclonal antibodies (D-07 and Y5) to the P53 protein, interacting with its “wild” and mutant isoforms. The significance of the difference in the samples was determined using the Mann-Whitney U-test, correlation relationships were determined using the Spearman coefficient. Results: Expression of P53 upon interaction with antibodies D-07 and Y5 was determined in 56.3% and 39.6%, respectively. A statistically significant direct correlation was found between the severity of P53 expression when interacting with Y5 antibodies and the degree of tumor differentiation (rs = 0.567, p <0.05), as well as between the expression level of this protein and tumor proliferative activity (rs = 0.698, p <0.05). Conclusion: Antibodies of clone D-07, interacting with both wild and mutant isoforms of P53 protein, show positive expression in adenocarcinomas of all degrees. Expression of the mutant P53 protein is most pronounced in low-differentiated carcinomas and correlates with high proliferative activity of tumor cells, which may be associated with a loss in the induction of P53-dependent apoptosis.
Other Latest Articles
- Polypathy: searching for etiopathogenetic risks factors
- Effects and clinical outcomes of early initiation of sacubitril/valsartan in patients with chronic heart failure with a low ejection fraction
- Advisable including glucosaminylmuramyldipeptide in Helicobacter pylori therapy: experience of ten-year investigation
- Main methods of psychotherapy for negative schizophrenia symptoms
- Physiological cost as a factor determining the effectiveness of human activity in the visual images reproduction
Last modified: 2020-09-10 05:19:59