Reversible Nematostatic Effect of Peganum harmala L. (Nitrariaceae) on Meloidogyne javanica

Meloidogyne javanicais considered as the most damaging nematode of vegetables in Morocco. Eco-friendly bionematicides are urgently required for its control. In vitroexperiments were carried out to assess the direct effect of bioproducts of P. harmalaagainst M. javanica. The bioassay showed extractstobenematotoxic.Aqueousextracts of P. harmala exhibited reversible nematostatic activity. The estimated ID50 of the most active product in methanolic extracts was 368 ppm. HPLC-MS of the methanolic etract revealed that total content ofmajoralkaloidsofP.harmalawasapproximately 12.162±0.637mg/g. Harmine (8.514 ± 0.521 mg/g) is the dominant alkaloid. In conclusion, P. harmalahas a reversible nematostaticactivityonsecondstagejuveniles of M. javanica. The effect of P. harmalaisduetoitspossessionofahighcontentofβ-carbolinealkaloids,which warrant further experimentation. Bioproducts from P. harmala should be exploitedthroughformulationsformanagement of the root knot nematode