Impact of PVC and Stainless-Steel Materials on Staphylococcus aureus Biofilms Production and their Thermodeactivation as Affected by pH, Time and NaCl

— Staphylococcus aureus is an opportunistic and ubiquitous pathogen capable of developing into a biofilm on work surfaces, processing surfaces or implants causing cross-contamination of food and humans. Heat treatment is the method that has shown effectiveness against the elimination of biofilms on work surfaces. However, the treatment is not always effective. The objective of this study was to evaluate the effect of some growth factors on the formation and thermo-deactivation of S. aureus biofilms on PVC and stainless-steel materials. S. aureus ATCC 13565 was firstly grown in nutrient broth at 37°C at different pH (5 – 7), NaCl (0,005-8%) and incubation duration (0-48h) following a CCD experimental design and their biofilm formation