ESTIMATION OF MOLECULAR DIVERSITY BETWEEN IMPORTANT NATIVE AND PURE FEMALE ASIATIC CHICKENS USING RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD)

RAPD was used to determine the molecular genetic diversity of four chicken species viz. White Plymouth Rock ? Rhode Island, commercial broiler and Aseel. Using eight oligo primers. a total 68 amplicons (average 8.5per primer) were obtained of which 51 polymorphic. Total 68 banding pattern were observed with an average of 7.6 per primer followed by 0.82% average discriminatory power, and 0.38 average PIC value. Based on UPGMA, Jaccard`s coefficient similarity range from 17 % (RIR2 and CB1) to 87 % (CB1 and CB2) Based on SHAN Cluster algorithms 12 accession grouped in four cluster group, Groups 1 with two accession (RIR2 and WPR2) group 2 with two accession (RIR1 WPR3) group 3 with 6 accession with two sp. Aseel and Commercial Broiler (AS1, AS2, AS3 CB1 CB2 and CB3 and group 4 with 2 accession (WPR1 and WPR2). In this study genetic relationships information generated through RAPD would be useful in utilization for future breeding purpose.