STUDY THE ANTIBACTERIAL ACTIVITY OF COPPER NANOPARTICLES SYNTHESIZED USING HERBAL PLANTS LEAF EXTRACTS

The plants Asparagus adscendens, Bacopa monnieri, Ocimum bacilicum, and Withania somnifera were used and compared for their extracellular synthesis of metallic copper nanoparticles (CuNPs). Stable Cu nanoparticles were formed by treating aqueous solution of CuSO4?5H2O with the plant leaf extracts as reducing agent. By treatment of 1mM aqueous solutions of CuSO4?5H2O with leaf extract stable CuNPs were formed; the change in color of solution confirm the formation of stable nanoparticles. UV-Visible study revealed qualitative formation of CuNPs and characteristic absorption peak in Asparagus adscendens, Bacopa monnieri and Ocimum bacilicum leaf extract at the range of 500-700nm but in Withania somnifera leaf extract absorption peak of CuNPs is shifted at the range of 500-800nm. These biosynthesized CuNPs were characterized with the help of Fourier transform infrared spectroscopy (FTIR), and Transmission Electron Microscopy (TEM). The involvement of primary and secondary metabolites or possible reducing agent confirmed by FTIR analysis. TEM confirms the formation and the crystalline nature of Copper nanomaterial. This method can be used as effective and environmental friendly technique for the synthesis of Copper nanoparticles using leaf extract of different plants. The antibacterial potentials of the CuNPs were studied and these are shown good antimicrobial activity against Gram positive and Gram negative bacteria.