METHOD DEVELOPMENT FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN AND NATEGLINIDE IN COMBINED DOSAGE FORM BY UV SPECTROSCOPY

In the present work, the quantitative estimation of both the drugs in combined dosage forms was carried out. A new, simple, reliable, sensitive, rapid, and economical procedure for simultaneous estimation of atorvastatin calcium and nateglinide in a combined dosage form by UV spectroscopy using the simultaneous equation method was developed. Native ultraviolet absorbance maxima of the two chemotherapeutic agents were used. As both compounds do not interact chemically in methanol, the two wavelengths 246.15 nm for atorvastatin calcium and 206.6 nm for nateglinide were used. Both the drugs obeyed Beer's law in the concentration range (1-10 µg/ml) that was employed in this method. The method developed was validated to determine its linearity (r2=0.996 for atorvastatin and r2=0.997 for nateglinide), precision, reproducibility, and sensitivity