ASSESSMENT OF HIGH YIELDING LOCAL PAKISTANI BREAD WHEAT GENOTYPES FOR IMPROVED CHAPATI MAKING QUALITY
Journal: World Journal of Biology and Biotechnology (Vol.5, No. 3)Publication Date: 2020-12-15
Authors : Jabeen Farheen Farzana Nasir Naqvi;
Page : 1-8
Keywords : cluster analysis; correlation analysis; genetic variability; SDS-PAGE; Triticum aestivum; glutenin protein.;
Abstract
The experiment was conducted to classify the maximum glutenin protein possessed Pakistani bread wheat genotype for superlative chapati making quality by ten yield-related parameters. The studied germplasm was acquired from NARC, Pakistan, and planted in randomized-complete-block-design with four replicates at the screen house of the Genetics Department. Data were assessed via Duncan's test, correlation analysis, SDS-PAGE, and cluster analysis. Duncan's test conceded that Pirsabak-85 had the highest plant height, flag leaf area, biomass, grain yield plantˉ1, harvest index, and protein content. While, the correlation studies showed that plant height, tillers plantˉ1 (r = 0.649), fertile tillers plantˉ1 (r = 0.713),biomass (r = 0.861), spike length (LS), thousand-grain weight and harvest index had a positive higher significant association with grain yield plantˉ1. The SDS-PAGE analysis resolved 30 diverse high and low molecular weight bands, ranging from 200 kDa to 28 kDa glutenin subunits. Among genotypes, Pirsabak-85 showed maximum protein content and 10 Glu-1 scores. The dendrogram analysis revealed that Pirsabak-85 associated with cluster-II, which was a major and most diverged cluster. The Pirsabak-85 can be utilized to enhance bread wheat production and better chapati making quality
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