Evaluation of Antioxidant Acitivity of Eisenia fetida

Antioxidant assay was performed to count the total phenolic content in coelomic fluid of Eisenia fetida. The worms were cultured in lab until they became sexually mature. The study focused on total phenolic compound determination using two solvents in a single species of earthworm. It was analysed that the extraction of phenolic compound with 85% ethanol yielded high phenol content, 208.6 mg GAEL Eisenia fetida. Extraction with 85% methanol on the other hand, yielded 189.1 mg GAEL. Eisenia fetida ethanolic extract in the sample manifested high total phenolic content than methanolic extract. Phenols are more readily soluble in ethanol and safe for human consumption. Methanol is used as solvent for low molecular weight polyphenols. The study concludes that ethanol extract of Eisenia fetida coelomic fluid contains significant amount of phenols. Phenols have an antioxidant effect and can be used as natural antioxidants for curing ailments related with inflammation and oxidative stress. This study will help in bringing attention towards the therapeutic utility of earthworms.