Expression of Immune Genes and Stress Enzyme Profiles of Rainbow Trout (Oncorhynchus mykiss) fed Moringa oleifera leaf meal (MLM)

Moringa oleifera belongs to family Moringaceae, is one in every of the foremost useful trees within the world, with all the parts having nutritional and pharmacological properties. This research evaluated the impact of categorized nutritive insertion of Moringa oleifera leaf meal (MLM) on survival, growth, and antioxidant systems, with a few immune-related organic phenomena in rainbow trout. Fish diets formulated (M0, M10, M20, M30, and M40) to contain 0, 10, 20, 30 and 40% of MLM as protein source supplemented with fish meal respectively. The rainbow trout fishes (6.57± 0.56 g) were randomly distributed into 15 plastic tanks (n=15) and fed the same (twice daily) for 90 days. Growth parameters (WG%, SGR, and FCR), serum protein contents (TSP, TSA, TSG, and A: G), enzyme contents (SOD, CAT, GPx, AST, ALT) and organic phenomena, IL-6, IL-8, IL-10, and TNF-α genes were studied, compared to β-actin genes followed by blood morphology of trout. Fish fed MLM accompanied diet showed higher weight gain (349.31%) and SGR (1.35) and minimum FCR (1.33) values up to M20 (20%) group of fish fed MLM diet compared with control fish. Protein profile in blood and enzyme-like SOD, CAT, and GPx found higher while lower AST and ALT recorded altogether the treated groups compared to regulate. Similarly, MLM supplemented diets fed fish exhibited the expression of immune genes. Interleukin -6, Interleukin -8 and Interleukin -10 found significantly high (P < 0.05) compared to β-actin gene. Finally, this research showed that dietary supplementation of 20% MLM is perfect for effective growth performance, enhancing the antioxidant activities and answering some immune performance in rainbow trout (Oncorhynchus mykiss).