BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES USING VICIA FABA EXTRACT AND ASSESSING ITS ANTIBACTERIAL ACTIVITY

The biological method provides a simple and efficient route for the synthesis of silver nanoparticles (AgNPs). The present study was aimed to synthesize AgNPs using Viciafaba seed extract as a reducing agent, which is a cost-effective, safe and eco-friendly method. The advantage of selecting this plant is that the phenolic and flavonoid compounds present in these seeds exhibit reducing property. Synthesized nanoparticles were characterized by using UV-Visible spectroscopy, Fe-SeM and X-ray diffractometer (XRD). Fe-SeM images showed that the particles were in the range of 12-22nm and flower structured. The formation and stability of AgNPs were confirmed by UV–vis spectrophotometer analysis, which showed higher absorbance at 430nm. XRD studies revealed a high degree of the crystalline structure of nanoparticles and a face-centered cubic structure. The anti-bacterial potency was tested against Escherichiacoli and Clostridium perfringens. These silver nanoparticles synthesized byV.faba seed extract exhibited the highest antibacterial effect against E.coli than C. perfringens.