Calculation on Fusion Stability on [DNA or RNA]- Peptide (FS) Algorithm “Cruz-Rodriguez “

During the last few decades, biotechnology has evolved into the construction of chemical chimeras of DNA/RNA fused to segments of proteins named peptides utilizing in this fusion a sequence of amino acids designated as spacing arm. This fusion is done with the goal of complementing or empowering the biological actions of one or both molecules, which, separately, present very low or null biological action, however, due to the fusion a functionally inert molecule could translate into a biochemically active tool. In the present work we present theoretical results of the ideal value of Fusion Stability (FS) at a value of 100%, measured in values of Cruz. For the calculation of FS=[abcd], we have assigned a constant value to one unit, the multiplication value of “bd”, where FS= 1*[ac]. In the linear equation it represents, the corresponding angle is ¥=45o . We have calculated the values for FS for an amplitude of ¥=45o in a variable range for the size Poly (A), primer size, size Poly Cys and the size of the peptide. We have reached the value FS=100 Cruz for the size 12 nucleotide (nt) of Poly (A), 8 aminoacid (aa) of size Poly Cys, 16 aa of size of the peptide and 24 nt size of primer [DNA or RNA]. We can conclude that the FS value is a predictive value of stability in Silico of a hybrid molecule [DNA or RNA]-peptide where the size of the peptide provides the greatest contribution to the accuracy of the FS value. Values greater than 60 Cruz are indicators in Silico of the chemical stability and biological functionality of a given chimera. This is of great value in the design of vaccines and conjugate medications.