Formulation, In-vitro and Ex-vivo Characterization Study of Frusemide Liposomal Drug Delivery System

Frusemide falls under Biological Classification System (BCS) IV. The aim of the study is to enhance permeation of the drug. In this study frusemide is entrapped by using aqueous solution (phosphate buffer pH 7.4) by the Phospholipid (PL) complex containing ethanol and Tween 80. Formulated liposome was carried out for optimization of the drug loading efficacy by using 12000 to 15000 Molecular Weight cut off of Dialysis Membrane. Central composite design was used to optimize formulation to increase drug loading. Contour plot of drug loading was drawnfrom the drug loading calculation as a primary response factor. Ex-vivo permeation study was carried out of the optimized formulation where inner part of the intestine is exposed towards the donor compartment of Franz diffusion cell. Tween 80 decreases the size of liposomal formulation forming small unilamelar vesicles that forms the most stable nanoliposome suspension. Three of the responded factors was noted in the experiment to degrade the stable product i.e. temperature in storage of extraction and formulation product and size of particles formed. The permeation study showed that there is 1.5 fold increase in flux of frusemide if it is used in liposomal dosage form.