Effect of Amlodipine Combined with N-acetylcysteine on Crohn’s Disease Model in Rats

This study highlights the effect of Amlodipine combined with N-acetylcysteine on Crohn's disease model in rats. Model of Crhon's disease was induced by subcutaneous Indomethacin administrated at a dose rate of 9 mg/kg for two days at 24h intervals. N-acetylcysteine (500mg/Kg), Amlodipine (10 mg/kg) and N-acetylcysteine (500 mg/kg) combined with Amlodipine (5mg/kg) were administrated for seven consecutive days beginning 24 h after the first Indomethacin injection. The small intestinal injury was assessed by small intestine weight/length ratio, macroscopic damage, histological study, as well as by biochemical measurement of reduced glutathione, lipid peroxides, and superoxide dismutase activity. The results showed that Administration of Amlodipine combined with N-acetylcysteine decreased small intestine weight/length ratio, macroscopic and microscopic small intestinal damage scores. Superoxide dismutase activity was increased; lipid peroxidation was decreased, but reduced glutathione levels were decreased in the combination group. No statistical significance was observed when previous findings were compared with Indomethacin control group (p>0.05). This study also showed that administration Amlodipine combined with N-acetylcysteine significantly decreased macroscopic score comparing with N-acetylcysteine administration alone. Conclude that combination of Amlodipine with N-acetylcysteine didn't produce obvious enhancement in the intestinal injury induced by Indomethacin (rat model of Crhon's disease), but this combination attenuated the injury comparing with N-acetylcysteine alone in this model of Crhon's disease.