Detection of the Presence of Trimethylamine in the Menstrual Blood of Women of Reproductive Age

The purpose of the study was to develop the criteria for establishing the origin of menstrual blood with the detection of trimethylamine in it by organoleptic and sensory methods. The article gives information on the diagnostic possibilities of detecting trimethylamine in the blood of menstrual origin on proprietary evidence by organoleptic and sensory methods. In expert practice, this method is completely new. The obtained results allow recommending it for forensic medical purposes, in particular, for determining the regional origin of menstrual blood. Materials and methods of research. Objects of research were dried on gauze menstrual blood, taken from practically healthy women of reproductive age and women with inflammatory diseases of the external genitalia. To establish the blood of menstrual origin, we removed from the dry objects of the study areas filled with menstrual blood in the size of 1.5x1.5 cm, added a few drops of 5-10% solution of potassium hydroxide (KOH). After 5-7 minutes, with a positive result, an unpleasant amine odor (rotten fish) was detected organoleptically, due to the presence of volatile amines, in particular trimethylamine, which provides the menstrual blood with a specific odor. Results and discussion. Due to this specific odor and the presence of menstrual origin blood it was determined on the basis of evidence. In parallel, experiments with spotted wine, ketchup, tomato and hawthorn flowers were also investigated, which was the appropriate test for comparison. We also used the technique of standard PANI preparation: oxidized 0.2 M aniline with 0.25 M ammonium peroxide sulfate in an aqueous medium. Anilin hydrochloride (2.59 g, 20 mmol) was dissolved in distilled water in a volumetric flask to 50 ml of solution. Ammonium peroxydisulfate (5.71 g, 25 mmol) was dissolved in water to 50 ml of solution. Both solutions were kept for one hour at 18-24° C, then mixed in a chemical glass, stirred and left until the next day. The next day, the precipitate was collected on a polymer carrier, washed in three portions of 100 ml of a 0.2 M solution of HCl, and the same with acetone. Polyaniline hydrochloride was dried in air, and then in a vacuum at 600° C. Additional polymerization was carried out in ice water at 0-2° C. The acidity of the reaction mixture was increased by replacing 10 ml of water, 10 ml of a 10 M solution of HCl in several experiments. Conclusions. The obtained results allow it to be recommended for forensic purposes, when determining the regional origin of blood, in particular menstrual blood.