Influence of Multiprobiotics on the Content of Nitrite Ions and the Activity of Nitric Oxide Synthase in Blood Serum and in the Mucous Membranes of the Stomach and Colon of Rats in Conditions of Prolonged Hypoacidity of Gastric Juice

Materials and methods. All animals were divided into 4 experimental groups. The first group of animals served as a control one. They were intraperitoneally administered (in/о) 0.2 mls and perorally (p/o) 0.5 mls of water for injections once a day during 28 days. The animals of the second group were administered omeprazole and p/o 0.5 mls of water for injections once every 24 hours during 28 days. The animals of the third group were administered omeprazole and multiprobiotic "Symbiter® acidophilic" concentrated (Symbiter) once every twenty-four hours during 28 days. The animals of the fourth group were administered omeprazole and multiprobiotic "Apibact®" (Apibact) once every twenty-four hours during 28 days. Results and discussion. The content of nitrite ions in the blood serum did not statistically differ from the control group at daily introduction of omeprazole with the multiprobiotic of "Symbiter" or with the multiprobiotic of "Apibact" during 28 days. In the mucous membrane of stomach the multiprobiotics of "Symbiter" and "Apibact" approximately identically diminished content of nitrite ions by 24.9% (p<0.05) and 20.2% (p<0.05), accordingly, in comparison to the group of rats that during 28th days was administered only omeprazole. Thus, the content of nitrite ions in the mucous membrane of stomach of rats remained high enough and was more than in control group by 83.9% (p<0.05) and 95.4% (p<0.05), accordingly. An analogical picture was observed in the colon. Increase of synthesis of NO2 was the result of increased activity of NOS. It is known that bacterial products in a gastrointestinal tract stimulate activity of іNOS of immunocompetency, ephithelial and other cells. Some bacteria are able to form NO from nitrogen-containing products. Determination of NOS activity in the mucous membranes of stomach and colon showed the increase in the rats mucous membranes of stomach and colon by 146.8% (р<0.05) and 113% (р<0.05) respectively, after 28 daily introductions of omeprazole. After the protracted compatible introduction of omeprazole+ multiprobiotic "Symbiter" and omeprazole+ multiprobiotic "Apibact", NOS activity diminished by 16.3% (p<0.05) and 14.4% (p<0.05), accordingly. As in the case of nitrite ions content, NOS activity did not recommence to the control values. Conclusions. The protracted oppression of secretion of hydrochloride acid in the stomach of rats caused the excessive generation of oxide of nitrogen in the mucous membranes of digestive tract. That showed up in the increase of NOS activity in the mucous membranes of stomach and colon by 146.8% (р<0.05) and 113% (р<0.05) respectively, and the simultaneous increase of concentration of NO2 in the blood serum in the mucous membranes of stomach and colon by 20% (р<0.05), 144% (р<0.05) and 158% (р<0.05) respectively.