SPECTROPHOTOMETRIC METHODS FOR QUANTITATIVE ESTIMATION OF TRAMADOL HYDROCHLORIDE FROM TABLET FORMULATION

Two simple and sensitive visible spectrophotometric methods have been developed for the quantitative estimation of Tramadol Hydrochloride from its tablet formulation. The developed methods are based on formation of chloroform extractable colored complex of drug with metanil yellow and tropaeolin ooo. The chloroform extracted complex of drug with metanil yellow showed absorbance maxima at 410.0 nm and linearity was observed in the concentration range of 3-24 μg/ml (method-I), with tropaeolin ooo showed absorbance maxima at 485.0 nm and linearity was observed in the concentration range of 3-24 μg/ml (method-II). Results of analysis for both the developed methods were validated statistically and by recovery studies.