DETECTION OF POLYMORPHISM IN FecB GENE IN BULGARIAN SHEEP BREEDS

The purpose of the present work was to identify allelic variants of ovine FecB gene in three fine fleece sheep breeds in Bulgaria – Askanian, Caucasian and Karnobat Merino. Blood samples were collected from 30 animals of each breed (total of 90 samples). Genomic DNA was extracted using commercial kit for DNA purification from whole blood. After PCR amplification of FecB gene with specific primer set a PCR fragment with length 190 bp was obtained. Genotypes were determined by restriction fragment length polymorphism (RFLP) method with AvaII restriction enzyme. The locus of Boroola gene was found to be monomorphic. Only allele ”+” was found and only genotype “++”, respectively. According to the results the investigated animals were non-carriers of the mutation.